85-4420-89　JMJD2C, Chemiluminescent, BioAssay™ Kit (Jumonji Domain Containing 2C, KDM4C, GASC1) 96Tests　298417

特徴

• JMJD2C is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.
• Intended Use:The JMJD2C Chemiluminescent BioAssay™ Kit is designed to measure activity of the JMJD2C for screening and profiling applications.
• Test Principle:The key to the JMJD2C Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2C enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
• Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high salt
• Applications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.
• Kit Components:Black microplate precoated with histone substrate, 1x plateJMJD2C (GASC1, KDM4C), 1x20ug Primary antibody 5, 1x25ul Secondary HRP-labeled antibody 1, 1x10ul4x JMJD2C assay buffer, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate, 2 components x 6ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.