85-4420-88　JARID1A, Chemiluminescent, BioAssay™ Kit (Jumonji/ARID Domain Containing Protein 1A, KDM5A, RBBP2) 96Tests　298416

特徴

• JARID1A is a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation.
• Intended Use:Great for studying enzyme kinetics and HTS applications.
• Test Principle:The JARID1A Chemiluminescent BioAssay™ Kit is designed to measure JARID1A activity for screening and profiling applications. The key to the JARID1A Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JARID1A enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
• Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high salt
• Kit Components:Black 8-well strip plate module precoated with histone substrate, 1x plateJARID1A (KDM5A, RBBP2), 1x10ug Primary antibody 13, 1x25ul Secondary HRP-labeled antibody 1, 1x10ul 4x JARID1A assay buffer, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate A, (transparent bottle), 1x6ml HRP chemiluminescent substrate B, (brown bottle), 1x6ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.