85-4420-71　CECR2 TR-FRET, BioAssay™ Kit (CECR2, Cat Eye Syndrome Chromosome Region Candidate 2, CECR-2) 1Kit　298398

特徴

• Kit comes in a convenient format with purified CECR2 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
• The CeCR2 TR-FRET BioAssay™ Kit is designed to measure the inhibition of CeCR2 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, CeCR2, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
• Applications:Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.
• Kit Components:White, Nonbinding, low volume, microtiter plate, 1x plateCeCR2, GST, 1x10ug BET Bromodomain Ligand, 1x50ulNon-acetylated Ligand 1, 1x15ul Tb donor, 2x10ul Dye-labeled acceptor, 2x10ul3x BRD TR-FRET Assay Buffer 1, 1x4ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.