The BRD4 (BD1) Inhibitor Screening BioAssay™ Kit is designed to measure the inhibition of BRD4 bromodomain 1 (BD1) from binding to its substrate. The key to the BRD4 (BD1) Inhibitor Screening BioAssay™ Kit is the highly specific binding of the BRD4 bromodomain 1 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD4 bromodomain 1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Applications:Suitable for use in the study of bromodomain binding assays, screening inhibitors andselectivity profiling. Other applications not tested.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
