This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010].
Source:Recombinant protein corresponding to aa65-187 from human Bromodomain containing 2, fused to His-tag at N-terminal, expressed in E. coli. Accession: NM_005104
Molecular Weight:~15.5kD
Applications:Suitable for use in the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Assay Conditions;BRD2 is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10ul reaction for 1 hour at RT. AlphaLISA® ® Nickel Chelate acceptor beads are added, followed by Alphascreen®® Streptavidin-conjugated donor beads. After 1 hour incubation at RT, Alphacounts are measured.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥98%
Form：Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 138mM sodium chloride, 2.7mM KCl, 0.05% Tween 20, 10% glycerol.
