85-4418-25　Epidermal Transglutaminase, Recombinant, Human (TGM3, TGe, Protein-Glutamine gamma-Glutamyltransferase E, EC=2.3.2.13,Transglutaminase E, Transglutaminase-3) 100ug　298170

特徴

• Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
• Source:Recombinant protein corresponding to human Epidermal Transglutaminase, expressed serum-free in a human cell line.
• Molecular Weight:~77kD
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:ELISA: coating antigen at 10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.