85-2663-25　Coelenterazine, native (Luciferin, Preluciferin) 10mg　CAS No:55779-48-1　257643

特徴

• Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.1 The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum, 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green or yellow fluorescent protein, in bioluminescent resonance energy transfer studies.2,3 Alternatively, the calcium-mediated release of coelenterazine from aequorin, followed by non-enzymatic oxidation of this compound, results in bioluminescence. As light emission depends on both calcium and cellular redox status, this reaction is used to non-fluorescently detect changes in calcium level and redox status.4,5
• Synonyms:3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one; CLZN; Preluciferin; Luciferin; 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one
• Molecular Formula:C₂₆H₂₁N₃O₃
• Molecular Weight:423.46
• Appearance:Brownish/yellow to yellow/orange crystalline powder
• Purity:≥95%
• Solubility (0.0125mg/ml in methanol:Orange to yellow to brown liquid
• Molar Extinction Coefficient (0.0125mg/ml in methanol, lambda max1):>7800.0 L/(mol cm)
• UV (0.0125mg/ml in methanol, lambda max1):431-435nm
• UV (0.0125mg/ml in methanol, lambda max2):261-265nm
• Storage and Stability:Store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic and light sensitive. Store in amber vial under inert atmosphere.
• Note:It is not recommended to store dissolved coelenterazine at -20°C or -70°C. Coelenterazine is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.