85-2103-36　Creatine Kinase (CK, Creatine Phosphokinase, Creatine N-Phosphotransferase, CPK, Phosphocreatine Phosphokinase) 25KU　CAS No:9001-15-4　C7910-01

特徴

• Creatine kinase (CK) is widely distributed and seems to be primarily concerned with ATP regeneration. CK may represent 10-20% of muscle cytoplasmic protein.
• Protein:≥ 0.6mg protein/mg (Coomassie)
• Activity:≥ 250 U/mg protein (Dimension® Clinical Chemistry System)
• Specific Activity:≥ 200U/mg protein
• Unit Definition:One Unit will transfer one micromole of phosphate from Creatine Phosphate to ADP per minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by a coupled reaction.
• Reconstitution:1mg protein/ml in Tris buffered saline, pH 8.0 containing 1mM dithiothreitol and 1mM EDTA. Add glycerol to 50% (v/v) and store at -20°C. Further dilutions should be in the reconstitution buffer plus 1% BSA as a carrier.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute and aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Store dessicated.