85-2103-03　［取扱停止］Vitamin A (VA), BioAssay™ ELISA Kit (Canine) 24Tests　228015

特徴

• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of endogenic canine Vitamin A (VA) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:1.56ng/ml
• Range:3.12-50ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of canine VA. No significant cross-reactivity or interference between canine VA and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VA antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (10X), 1x200ul Sample Diluent, 2x20ml HRP-conjugate (100X), 1x60ul HRP-conjugate Diluent, 1x10ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.