85-2102-91　［取扱停止］Vascular Endothelial Cell Growth Factor Receptor 2, BioAssay™ ELISA Kit (Human) (VEGFR-2, Flk-1, CD309, FLK1, VEGFR, VEGFR2, Fetal Liver Kinase-1, Kinase Insert Domain Receptor, Protein-Tyrosine Kinase Receptor Flk-1, Soluble VEGFR2, Tyrosine Kinase Growth Factor Receptor) 48Tests　228012

特徴

• Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alpha V beta 3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of human vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:0.098ng/ml
• Range:0.390-25ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human VEGFR-2/Flk-1. No significant cross-reactivity or interference between human VEGFR-2/Flk-1 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for VEGFR-2/Flk-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFR-2/Flk-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFR-2/Flk-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFR-2/Flk-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.