85-2102-90　［取扱停止］Vascular Cell Adhesion Molecule 1, BioAssay™ ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen) 24Tests　228011

特徴

• This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
• Sample Type:Serum, plasma, cell culture supernates and tissue homogenates
• Intended Use:For the quantitative determination of mouse vascular cell adhesion molecule 1 (VCAM-1) concentrations in serum, plasma, cell culture supernates and tissue homogenates.
• Sensitivity:29.649pg/ml
• Range:39.062-2500pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of mouse VCAM-1. No significant cross-reactivity or interference between mouse VCAM-1 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for VCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.