85-2102-35　［取扱停止］Transforming Growth Factor Beta 1, BioAssay™ ELISA Kit (Human) (TGF-b1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 Protein, Latency-Associated Peptide) 96Tests　227993

特徴

• Sample Type:Serum, plasma and cell culture supernates
• Intended Use:For the quantitative determination of human transforming growth factor beta 1 (TGF-b1) concentrations in serum, plasma and cell culture supernates.
• Sensitivity:0.747ng/ml
• Range:0.78-50ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human TGF-b1. No significant cross-reactivity or interference between human TGF-b1 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-b1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-b1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-b1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-b1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml 1N HCI, 1x10ml1.2N NaOH/0.5M HEPES, 1x10mlAdhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.