85-2102-32　［取扱停止］Transferrin, BioAssay™ ELISA Kit (Human) (TF, PRO1557, PRO2086) 96Tests　227992

特徴

• This gene encodes a glycoprotein with an approximate molecular weight of 76.5kD. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.
• Sample Type:Serum, plasma, urine, cell culture supernates and cerebrospinal fluid (CSF)
• Intended Use:For the quantitative determination of human transferrin (TF) concentrations in serum, plasma, urine, cell culture supernates and cerebrospinal fluid (CSF).
• Sensitivity:0.006nmol/L
• Range:0.023-1.5nmol/L
• Specificity:This assay has high sensitivity and excellent specificity for detection of human TF. No significant cross-reactivity or interference between human TF and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TF present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for TF is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TF bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vialsHRP-conjugate (100X), 1x120ulHRP-conjugate Diluent, 1x20mlSample Diluent, 2x20mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.