85-2102-25　［取扱停止］Toxoplasmosis (TOXO) Antibody, IgG, BioAssay™ ELISA Kit (Sheep) 48Tests　227982

特徴

• Sample Type:Serum and plasma
• Intended Use:This immunoassay kit allows for the in vitro semi-quantitative determination of sheep anti-toxoplasmosis (TOXO) antibody (IgG) concentrations in serum and plasma.
• Specificity:This assay recognizes sheep anti-toxoplasmosis (TOXO) antibody (IgG). No significant cross-reactivity or interference was observed.
• Test Principle:The microtiter plate provided in this kit has been pre-coated with specific antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-sheep IgG to each well and incubate. Finally, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. Calculate the valence of sheep anti-toxoplasmosis (TOXO) antibody (IgG) in the samples.
• Kit Components:Assay plate, 1x96 wellsSample Diluent, 2x20mlHRP-Conjugate Diluent, 1x10mlHRP-Conjugate (1:100), 1x120ulWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlPositive Control, 1x800ulNegative Control, 1x800ul
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.