85-2101-28　Testosterone (T), BioAssay™ ELISA Kit (Mouse) 48Tests　227938

特徴

• Testosterone (T), BioAssay™ ELISA Kit (Mouse) is a competitive ELISA intended for the quantitative determination of endogenic mouse testosterone concentrations in serum, plasma and tissue homogenates.
• Detection Range:0.1-20ng/ml
• Sensitivity:The minimum detectable dose of mouse testosterone is typically less than 0.05ng/ml
• The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse testosterone concentration that could be differentiated from zero.
• Specificity:This assay has high sensitivity and excellent specificity for detection of mouse Testosterone. No significant crossreactivity or interference between mouse testosterone and analogs was observed
• Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between mouse Testosterone and all the analogs. Therefore, crossreaction may still exist.
• Kit Components:227938A: Microtiter Strips, 1x96 wells227938B: Standard, 0ng/ml, 1x500ul227938C: Standard, 0.1ng/ml, 1x500ul227938D: Standard, 0.4ng/ml, 1x500ul227938E: Standard, 1.6ng/ml, 1x500ul227938F: Standard, 5ng/ml, 1x500ul227938G: Standard, 20ng/ml, 1x500ul227938H: Antibody 1x6ml227938J: HRP Conjugate, 1x6ml227938K: Wash Buffer, 20X, 1x15ml227938L: Substrate A, 1x7ml227938M: Substrate B, 1x7ml227938N: Stop Solution, 1x7ml
• Note: The Stop Solution (227938N) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Assay Summary:1. Prepare reagents and samples as instructed
• 2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well
• 4. Add 50ul HRP-conjugate to each well (Not to the Blank well)
• 5. Add 50ul Antibody to each well
• 6. Incubate for 60 minutes at 37°C
• 7. Aspirate and wash 3 times
• 8. Add 50ul Substrate A and 50ul Substrate B to each well
• 9. Incubate for 15 minutes at 37°C. Protect from light
• 10. Add 50ul Stop Solution to each well. Read at 450nm within 10 minutes.