85-2100-79　［取扱停止］Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO), BioAssay™ ELISA Kit (Plant) 48Tests　227904

特徴

• Ribulose-1,5-bisphosphate carboxylase oxygenase, most commonly known by the shorter name RuBisCO, is an enzyme involved in the Calvin cycle that catalyzes the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as glucose. RuBisCO catalyzes either the carboxylation or the oxygenation of ribulose-1,5-bisphosphate (also known as RuBP) with carbon dioxide or oxygen. RuBisCO is very important in terms of biological impact because it catalyzes the primary chemical reaction by which inorganic carbon permanently enters the biosphere. Many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle or the reverse Krebs cycle, but they make up a relatively minor portion of global net primary production. Phosphoenolpyruvate carboxylase PEPC only temporarily fixes carbon. RuBisCO is also the most abundant protein in leaves, and is considered to be the most abundant protein on Earth.It accounts for 50% of soluble leaf protein in C3 plants (20-30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5-9% of total leaf nitrogen).Given its important role in the biosphere, there are currently efforts to genetically engineer crop plants so as to contain more efficient RuBisCO.
• Sample Type:Plant tissues
• Intended Use:For the quantitative determination of endogenic ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) concentrations in plant tissues.
• Sensitivity:1.56ug/ml
• Range:3.12-800ug/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO. The competitive inhibition reaction is launched between with pre-coated RuBisCO and RuBisCO in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard, 2x500ul Antibody (100X), 1x60ul HRP-conjugate (100X), 1x120ul Antibody Diluent, 1x10ml HRP-conjugate Diluent, 1x20ml Sample Diluent, 2x20ml Sample Extraction Buffer (25X), 1x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.