85-2100-64　［取扱停止］Prostate Specific Antigen, BioAssay™ ELISA Kit (Human) (PSA, APS, KLK2A1, HK3, P-30 Antigen, Gamma-Seminoprotein, Semenogelase, Seminin) 48Tests　227890

特徴

• Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
• Sample Type:Serum, plasma, cell culture supernates and tissue homogenates
• Intended Use:For the quantitative determination of human prostate specific antigen (PSA) concentrations in serum, plasma, cell culture supernates and tissue homogenates.
• Sensitivity:0.5ng/ml
• Range:1-32ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human PSA. No significant cross-reactivity or interference between human PSA and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PSA. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSA bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard, 5x0.5ml HRP-conjugate, 1x6ml Substrate A, 1x7ml Substrate B, 1x7ml Stop Solution, 1x7ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.