85-2100-21　［取扱停止］Platelet Factor 4, BioAssay™ ELISA Kit (Mouse) (PF-4, CXCL4, SCYB4, Chemokine (C-X-C Motif) Ligand 4, Platelet Factor 4 (Chemokine (C-X-C Motif) Ligand 4)) 24Tests　227870

特徴

• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of mouse platelet factor 4 (PF-4) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:1.603ng/ml
• Range:3.125-200ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of mouse PF-4. No significant cross-reactivity or interference between mouse PF-4 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.