85-2099-91　Papillomavirus Type 16 (HPV16) Antibody, IgM, BioAssay™ ELISA Kit (Human) 48Tests　227852

特徴

• Sample Type:Serum and plasma
• Intended Use:For the qualitative determination of human papillomavirus type 16 (HPV16) antibody (IgM) concentrations in serum and plasma.
• Specificity:This assay has high sensitivity and excellent specificity for detection of human HPV16 antibody (IgM). No significant cross-reactivity or interference between human HPV16 antibody (IgM) and analogues was observed.
• Test Principle:This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HPV16 antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Coated assay plate, 1x96 wellsNegative Control, 1x800ul Positive Control, 1x800ul HRP-conjugate (100X), 1x120ul HRP-conjugate Diluent, 1x20ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.