85-2099-63　［取扱停止］Microalbuminuria, BioAssay™ ELISA Kit (Human) (MAU, ALB) 96Tests　227813

特徴

• Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
• Sample Type:Serum, plasma and urine
• Intended Use:For the quantitative determination of human Microalbuminuria (MAU/ALB) concentrations in serum, plasma and urine.
• Sensitivity:0.5ug/ml
• Range:1-50ug/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human MAU. No significant cross-reactivity or interference between human MAU and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. An antibody specific for MAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated MAU. A competitive inhibition reaction is launched between MAU (standards or samples) and HRP-conjugated MAU with the pre-coated antibody specific for MAU. The more amount of MAU in samples, the less antibody bound by HRP-conjugated MAU. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of MAU in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard, 5x1ml HRP-conjugate, 1x6ml Wash Buffer (20X), 1x15ml Substrate A, 1x7ml Substrate B, 1x7ml Stop Solution, 1x7ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.