85-2099-52　［取扱停止］Matrix Metalloproteinase 9, BioAssay™ ELISA Kit (Human) (Gelatinase B, MMP-9, CLG4B, GELB, MANDP2, Macrophage Gelatinase, Matrix Metalloproteinase 9 (Gelatinase B, 92kD Gelatinase, 92kD Type IV Collagenase), Type V Collagenase) 24Tests　227808

特徴

• Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and urine
• Intended Use:For the quantitative determination of human matrix metalloproteinase 9/gelatinase B (MMP-9) concentrations in serum, plasma, tissue homogenates, cell culture supernates and urine.
• Sensitivity:0.284ng/ml
• Range:0.312-20ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human MMP-9. No significant cross-reactivity or interference between human MMP-9 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.