85-2099-40　［取扱停止］Macrophage-Derived Chemokine, BioAssay™ ELISA Kit (Mouse) (MDC, CCL22, A-152E5.1, ABCD-1, DC/B-CK, MDC, SCYA22, STCP-1, CC Chemokine STCP-1, Macrophage-Derived Chemokine, Small Inducible Cytokine A22, Small Inducible Cytokine Subfamily A (Cys-Cys), Member 22) 24Tests　227800

特徴

• This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology.
• Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and cell lysates
• Intended Use:For the quantitative determination of mouse macrophage-derived chemokine (MDC) concentrations in serum, plasma, tissue homogenates, cell culture supernates and cell lysates.
• Sensitivity:7.8pg/ml
• Range:31.25-2000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of mouse MDC. No significant cross-reactivity or interference between mouse MDC and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for MDC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDC bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X concentrate), 1x120ul HRP-avidin (100X concentrate), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X concentrate), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.