85-2099-08　［取扱停止］Kidney Injury Molecule 1, BioAssay™ ELISA Kit (Rat) (Kim-1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1, T Cell Immunoglobin Domain and Mucin Domain Protein 1) 48Tests　227775

特徴

• Sample Type:Serum, plasma and urine
• Intended Use:For the quantitative determination of rat kidney injury molecule 1 (Kim-1) concentrations in serum, plasma and urine.
• Sensitivity:0.228ng/ml
• Range:0.313-20ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of rat Kim-1. No significant cross-reactivity or interference between rat Kim-1 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Kim-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Kim-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Kim-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Kim-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.