85-2099-01　［取扱停止］Japanese Encephalitis (JE) Antibody, IgM, BioAssay™ ELISA Kit (Human) 24Tests　227772

特徴

• Sample Type:Serum
• Intended Use:For the qualitative determination of human Japanese encephalitis antibody (IgM) concentrations in serum.
• Specificity:This assay has high sensitivity and excellent specificity for detection of human Japanese encephalitis antibody (IgM). No significant cross-reactivity or interference between human Japanese encephalitis antibody (IgM) and analogues was observed.
• Test Principle:This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human Japanese encephalitis antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Coated assay plate, 1x96 wellsNegative Control, 1x0.5ml Positive Control, 1x0.5ml Sample Diluent, 1x12ml HRP-conjugate, 1x12ml Wash Buffer (20X), 1x15ml Substrate A, 1x7ml Substrate B, 1x7ml Stop Solution, 1x7ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.