85-2098-91　［取扱停止］Interleukin 8, BioAssay™ ELISA Kit (Human) (IL-8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T Cell Chemotactic Factor, Beta-Thromboglobulin-Like Protein, Chemokine (C-X-C Motif) Ligand 8, Emoctakin, Granulocyte Chemotactic Protein 1) 24Tests　227769

特徴

• This protein is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
• Sample Type:Serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates and cell lysates
• Intended Use:For the quantitative determination of human interleukin 8 (IL-8) concentrations in serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates and cell lysates.
• Sensitivity:7.110pg/ml
• Range:31.25-2000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human IL-8. No significant cross-reactivity or interference between human IL-8 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.