85-2098-85　［取扱停止］Interleukin 18, BioAssay™ ELISA Kit (Human) (IL-18, IGIF, IL-1g, IL1F4, IL-1 Gamma, Interferon-Gamma-Inducing Factor, Interleukin-1 Gamma, Interleukin-18) 24Tests　227766

特徴

• This protein is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
• Sample Type:Serum, plasma, urine and tissue homogenates
• Intended Use:For the quantitative determination of human interleukin 18 (IL-18) concentrations in serum, plasma, urine and tissue homogenates.
• Sensitivity:1.95pg/ml
• Range:7.8-500pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human IL-18. No significant cross-reactivity or interference between human IL-18 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.