85-2098-41　［取扱停止］Immunoglobulin G (IgG), BioAssay™ ELISA Kit (Porcine) 48Tests　227737

特徴

• Sample Type:Serum, plasma, cell culture supernates and tissue homogenates
• Intended Use:For the quantitative determination of porcine immunoglobulin G (IgG) concentrations in serum, plasma, cell culture supernates and tissue homogenates.
• Sensitivity:0.58ug/ml
• Range:0.58-150ug/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of porcine IgG. No significant cross-reactivity or interference between porcine IgG and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with porcine IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for porcine IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of porcine IgG in samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard, 6x0.5ml HRP-conjugate, 1x6ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.