85-2098-28　［取扱停止］Equine IgG (Immunoglobulin G) BioAssay™ ELISA Kit 24Tests　227732

特徴

• The Equine IgG (Immunoglobulin G) BioAssay™ ELISA Kit is a competitive ELISA intended for the quantitative determination of IgG concentration in equine serum and plasma.
• Detection Range:25-400ug/ml
• Sensitivity:<23.764ug/ml
• Precision: Intra-Assay CV: <8%Inter-Assay CV: <10%
• Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with equine IgG. Standards, samples and HRP-conjugated anti-equine IgG are added to the appropriate microtiter plate wells and a competitive reaction is launched between plate-coated equine IgG and equine IgG in the standards or samples for limited antibody binding sites on the HRP-conjugated anti-equine IgG reagent. The wells are washed to remove unbound materials. TMB substrate solution is then added to the wells; color development is stopped and absorbance read at 450nm wavelength. The intensity of the color is inversely proportional to the amount of equine IgG in the samples.
• Kit Components:227665A: Microtiter Strips, 1x96 wells. Pre-coated; ready to use. 227665B: Standard, 0ug/ml, 1x500ul227665C: Standard, 25ug/ml, 1x500ul227665D: Standard, 50ug/ml, 1x500ul227665E: Standard, 100ug/ml, 1x500ul227665F: Standard, 200ug/ml, 1x500ul227665G: Standard, 400ug/ml, 1x500ul227665H: HRP Conjugate, 1x6ml227665J: Sample Diluent 2x20ml227665K: Wash Buffer, 25X, 1x20ml227665L: TMB Substrate, 1x10ml227665M: Stop Solution, 1x10ml
• Note: The Stop Solution (227732K) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Assay Summary:1. Prepare reagents and samples as instructed
• 2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well
• 4. Add 50ul HRP Conjugate to each well (do not add any to the blank well)
• 5. Incubate for 30 minutes at 37°C
• 6. Aspirate and wash 5 times
• 7. Add 90ul TMB Substrate to each well
• 8. Incubate for 20 minutes at 37°C. Protect from light9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.