85-2097-87　Hepatitis D Virus (HDV) Antigen, BioAssay™ ELISA Kit (Human) 96Tests　227714

特徴

• The Hepatitis D Virus (HDV) Antigen, BioAssay™ ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of human Hepatitis D virus (HDV) antigen in serum and plasma.
• Test Principle:This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase (HRP). Any HDV antigen present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human hepatitis D virus (HDV) antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Specificity:This assay has high sensitivity and excellent specificity for detection of human hepatitis D virus (HDV) antigen. No significant cross-reactivity or interference between human hepatitis D virus (HDV) antigen and analogues was observed.
• Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between human HDV and all the analogs. Therefore, crossreaction may still exist.
• Precision:Intra-assay Precision (Precision within an assay) %CV: <15%Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision (Precision between assays) %CV: <15%Three samples of known concentration were tested in twenty assays to assess.
• Kit Components:227714A: Microtiter Strips, 1x24 wells, 1x48 wells or 1x96 wells227714B: Negative Control, 1x80ul, 1x160ul or 1x300ul227714C: Positive Control, 1x80ul, 1x160ul or 1x300ul227714D: Antigen Lysate Buffer, 1x1.5ml, 1x3ml or 1x6ml227714E: HRP Conjugate, 1.5ml, 1x3ml or 1x6ml227714F: Wash Buffer, 30X, 1x15ml or 1x30ml227714G: Substrate A, 1.5ml, 1x3ml or 1x6ml227714H: Substrate B, 1.5ml, 1x3ml or 1x6ml227714J: Stop Solution, 1.5ml, 1x3ml or 1x6ml
• Note: The Stop Solution (227714J) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.