85-2097-33　［取扱停止］Haptoglobin, BioAssay™ ELISA Kit (Human) (Hpt, HP, BP, HP2-Alpha-2, HPA1S, Binding Peptide, Haptoglobin alpha(1S)-beta, Haptoglobin alpha(2FS)-beta, Haptoglobin alpha Polypeptide, Haptoglobin beta Polypeptide) 96Tests　227695

特徴

• This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn’s disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson’s disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
• Sample Type:Serum, plasma and urine
• Intended Use:For the quantitative determination of human haptoglobin (Hpt/HP) concentrations in serum, plasma and urine.
• Sensitivity:1.044ng/ml
• Range:1.563-100ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human Hpt. No significant cross-reactivity or interference between human Hpt and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Hpt has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hpt present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for Hpt is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hpt bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (100X), 1x100ulHRP-conjugate (100X), 1x120ulHRP-conjugate Diluent, 1x20mlSample Diluent, 2x20mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.