85-2097-28　［取扱停止］Growth Arrest-Specific Gene-6, BioAssay™ ELISA Kit (Mouse) (Gas-6, AXLLG, AXSF, AXL Receptor Tyrosine Kinase Ligand, AXL Stimulatory Factor) 24Tests　227693

特徴

• This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of mouse growth arrest-specific gene-6 (gas-6) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:17.086pg/ml
• Range:31.25-2000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of mouse gas-6. No significant cross-reactivity or interference between mouse gas-6and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for gas-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any gas-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for gas-6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of gas-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.