85-2096-47　［取扱停止］Fc Region of Immunoglobulin G (Fcg), BioAssay™ ELISA Kit (Human) 48Tests　227654

特徴

• Sample Type:Serum and plasma
• Intended Use:For the quantitative determination of human Fc region of immunoglobulin G (Fcg) concentrations in serum and plasma.
• Sensitivity:0.027ug/ml
• Range:0.027-20ug/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human Fcg. No significant cross-reactivity or interference between human Fcg and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fcg. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to Fcg. The competitive inhibition reaction is launched between with pre-coated Fcg and Fcg in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Fcg in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Sample Diluent, 2x20ml HRP-conjugate (100X concentrate), 1x60ul HRP-conjugate Diluent, 1x20ml Wash Buffer (25X concentrate), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.