85-2095-99　［取扱停止］Epidermal Growth Factor Receptor, BioAssay™ ELISA Kit (Human) (EGFR, ERBB, ERBB1, HER1, PIG61, MENA, Avian Erythroblastic Leukemia Viral (v-erb-b) Oncogene Homolog, Cell Growth Inhibiting Protein 40, Cell Proliferation-Inducing Protein 61) 96Tests　227630

特徴

• This protein is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of human epidermal growth factor receptor (EGFR) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:0.019ng/ml
• Range:0.312-20ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human EGFR. No significant cross-reactivity or interference between human EGFR and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X concentrate), 1x120ul HRP-avidin (100X concentrate), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X concentrate), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.