85-2095-08　［取扱停止］Cortisol (COR), BioAssay™ ELISA Kit 48Tests　227572

特徴

• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of cortisol (COR) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:0.156ng/ml
• Range:0.049ng/ml-200ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of COR. No significant cross-reactivity or interference between COR and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for COR and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated COR and COR in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of COR in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vials Antibody (100X concentrate), 1x60ul Antibody Diluent,1x10ml HRP-conjugate (100X concentrate), 1x120ul HRP-conjugate Diluent, 1x20ml Sample Diluent, 2x20ml Wash Buffer (25X concentrate), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.