85-2095-06　［取扱停止］Complement 3 (C3), BioAssay™ ELISA Kit (Human) 96Tests　227571

特徴

• Sample Type:Serum, plasma, cell culture supernates, urine and saliva
• Intended Use:For the quantitative determination of human complement 3 (C3) concentrations in serum, plasma, cell culture supernates, urine and saliva.
• Sensitivity:4.031ng/ml
• Range:4.7-300ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human C3. No significant cross-reactivity or interference between human C3 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for C3 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vialsSample Diluent, 2x20mlHRP-conjugate (100X concentrate), 1x120ulHRP-conjugate Diluent, 1x20mlWash Buffer (25X concentrate), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.