85-2094-35　［取扱停止］Arachidonic Acid (AA), ELISA Kit (Sheep) 48Tests　227532

特徴

• Sample Type:Serum, plasma, tissue homogenates
• Intended Use:This immunoassay kit allows for the in vitro quantitative determination of sheep AA concentrations in serum, plasma, tissue homogenates. This kit is designed to specifically recognize endogenic AA in sheep fluid.
• Sensitivity:15.6ng/ml
• Range:62.5-1000ng/ml
• Specificity:Recognizes sheep AA. No significant cross-reactivity or interference was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. An antibody specific to AA has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated AA and incubated. A competitive inhibition reaction is launched between AA (Standards or samples) and HRP-conjugated AA with the pre-coated antibody specific for AA. The more amount of AA in samples, the less antibody bound by HRP-conjugated AA. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of AA in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x plateStandard, 2x vials Sample Diluent, 2x20mlHRP-conjugate Diluent, 1x10mlHRP-conjugate, 1x60ulWash Buffer, 25X, 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.