85-2094-13　［取扱停止］Apolipoprotein E, BioAssay™ ELISA Kit (Mouse) (Apo-E, AD2, LDLCQ5, LPG, Apolipoprotein E3) 24Tests　227524

特徴

• Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
• Sample Type:Serum, plasma, tissue homogenates and cell culture supernates
• Intended Use:For the quantitative determination of mouse apolipoprotein E (Apo-E) concentrations in serum, plasma, tissue homogenates and cell culture supernates.
• Sensitivity:1.56ng/ml
• Range:6.25-400ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of mouse Apo-E. No significant cross-reactivity or interference between mouse Apo-E and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-E. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-E. The competitive inhibition reaction is launched between with pre-coated Apo-E and Apo-E in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-E in the samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vialsHRP-conjugate (100X concentrate), 1x60ulHRP-conjugate Diluent, 1x10mlSample Diluent, 2x20mlWash Buffer (25X concentrate), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.