85-2093-91　［取扱停止］Apolipoprotein E, BioAssay™ ELISA Kit (Bovine) (APOE, Apo-E, AD2, LDLCQ5, LPG, Apolipoprotein E3) 24Tests　227517

特徴

• Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of bovine apolipoprotein E (APOE) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:7.81ng/ml
• Range:31.25-2000ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of bovine APOE. No significant cross-reactivity or interference between bovine APOE and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOE. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOE. The competitive inhibition reaction is launched between with pre-coated APOE and APOE in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOE in the samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vialsHRP-conjugate (100X concentrate), 1x60ulHRP-conjugate Diluent, 1x10mlSample Diluent, 2x20mlWash Buffer (25X concentrate), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.