85-2093-83　［取扱停止］Apolipoprotein B100, BioAssay™ ELISA Kit (Human) (Apo-B100, FLDB, LDLCQ4, ApoB-100, ApoB-48, Apolipoprotein B, Apolipoprotein B48) 48Tests　227515

特徴

• This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
• Sample Type:Serum, plasma, cell culture supernates, saliva and urine
• Intended Use:For the quantitative determination of human apolipoprotein B100 (Apo-B100) concentrations in serum, plasma, cell culture supernates, saliva and urine.
• Sensitivity:19.5ng/ml
• Range:78-5000ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human Apo-B100. No significant cross-reactivity or interference between human Apo-B100 and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-B100. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-B100. The competitive inhibition reaction is launched between with pre-coated Apo-B100 and Apo-B100 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-B100 in the samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vialsHRP-conjugate (100X concentrate), 1x60ulHRP-conjugate Diluent, 1x10mlSample Diluent, 2x20mlWash Buffer (25X concentrate), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.