85-2093-80　［取扱停止］Apolipoprotein B100 (Apo-B100), BioAssay™ ELISA Kit (Rabbit) 48Tests　227514

特徴

• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of rabbit apolipoprotein B100 (Apo-B100) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:19.5ng/ml
• Range:78-5000ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of rabbit Apo-B100. No significant cross-reactivity or interference between rabbit Apo-B100 and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-B100. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-B100. The competitive inhibition reaction is launched between with pre-coated Apo-B100 and Apo-B100 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-B100 in the samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vialsSample Diluent, 2x20mlHRP-conjugate (100X concentrate), 1x60ulHRP-conjugate Diluent, 1x10mlWash Buffer (25X concentrate), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.