85-2093-45　［取扱停止］Apolipoprotein A1, BioAssay™ ELISA Kit (Human) (Apo-A1) 96Tests　227502

特徴

• This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.
• Sample Type:Serum, plasma, cell culture supernates, saliva and urine
• Intended Use:For the quantitative determination of human apolipoprotein A1 (Apo-A1) concentrations in serum, plasma, cell culture supernates, saliva and urine.
• Sensitivity:0.7ng/ml
• Range:1.4-1000ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human Apo-A1. No significant cross-reactivity or interference between human Apo-A1and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A1 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for Apo-A1 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96 wellsStandard (Freeze dried), 2x vialsHRP-conjugate (100X concentrate), 1x120ulHRP-conjugate Diluent, 1x20mlSample Diluent, 2x20mlWash Buffer (25X concentrate), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.