85-2093-33　［取扱停止］Angiogenin, BioAssay™ ELISA Kit (Human) (ANG, ALS9, HEL168, RNASE4, RNASE5, Epididymis Luminal Protein 168) 96Tests　227497

特徴

• This protein is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5 exons. Each gene splices to a unique downstream exon that contains its complete coding region.
• Sample Type:Serum, plasma, cell culture supernates, urine and tissue homogenates
• Intended Use:For the quantitative determination of human angiogenin (ANG) concentrations in serum, plasma, cell culture supernates, urine and tissue homogenates.
• Sensitivity:3.9pg/ml
• Range:15.6-1000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human ANG. No significant cross-reactivity or interference between human ANG and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X concentrate), 1x120ul HRP-avidin (100X concentrate), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X concentrate), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.