85-2092-56　Abscisic Acid, Hormone (ABA), BioAssay™ ELISA Kit (Plant) 48Tests　227466

特徴

• The Abscisic Acid, Hormone (ABA), BioAssay™ ELISA Kit is a competitive ELISA intended for the quantitative determination of ABA in plant tissues.
• Detection Range:0.156-10ug/ml
• Sensitivity:<0.04ug/ml
• Precision:Intra-assay Precision: CV <10% Inter-assay Precision: CV <20%
• Specificity:Cross reaction rate: Abscisic acid: 100%, Gibberellin and Indoleacetic acid: <0.01%
• Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with ABA. Standards and samples are added to the appropriate microtiter plate wells with an antibody specific to ABA. The competitive inhibition reaction is launched between the pre-coated ABA and ABA in samples with the antibody. Then an IgG (HRP) goat anti-rabbit antibody is added. A substrate solution is added to the wells and the color develops inversely to the amount of ABA in the sample. The color development is stopped and the intensity of the color is measured.
• Kit Components:227466A: Microtiter Strips, 1x48 wells or 1x96 wells227466B: Standard, 10X, 1x100ul or 1x200ul227466C: ABA Antibody, 100X, 1x30ul or 1x60ul227466D: HRP Conjugate, 100X, 1x60ul or 1x120ul227466E: Antibody Diluent, 1x10ml or 1x20ml227466F: HRP Diluent, 1x10ml or 1x20ml227466G: Sample Extraction Buffer, 25X, 1x20ml227466H: Sample Diluent, 1x20ml or 1x40ml227466J: Wash Buffer, 25X, 1x20ml 227466K: TMB Substrate, 1x10ml or 1x20ml227466L: Stop Solution, 1x10ml or 1x20ml
• Note: The Stop Solution (227466L) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.