85-2092-33　［取扱停止］Osteocalcin (1-49) (Gla17,21,24) 500ug　227382

特徴

• Osteocalcin (OC) is a 49 amino acid peptide found exclusively in bone tissue and ishighly conserved among species. It is a vitamin K- and D-dependent protein produced byosteoblasts, osteocytes and odontoblasts. It is deposited in extracellular bone matrix and is found in the serum. Serum osteocalcin, hydrolysed in the kidney and liver, is considered a specific marker of osteoblast activity and bone formation rate. It may be involved in regulation of osteoblast function, regulation of bone turnover and/or mineralization.
• Amino Acid Sequence:Tyr-Leu-Tyr-Gln-Trp-Leu-Gly-Ala-Pro-Val-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Arg-Arg-Gla-Val-Cys-Gla-Leu-Asn-Pro-Asp-Cys-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val (Gla=y-Carboxyglutamic Acid; Disulfide bridge: Cys23-Cys29)
• Reconstitution: Use 1.0% NH4OH as the solvent, followed by buffer (i.e.1X PBS). Add 1.0% NH4OH directly to the lyophilized peptide powder (add 35-40ul to 0.5mg peptide or 70-80ul to 1mg peptide). The peptide cannot be stored long term in 1.0% NH4OH, and it is therefore important to immediately dilute this solution with 1X PBS or other buffer to a concentration of ~1mg/ml or less. Gently vortex to mix.
• Storage and Stability:Store lyophilized powder -20°C. Powder is stable at -20°C for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Reconstituted peptide should be aliquoted into single use vials and stored at 20°C. Do not freeze-thaw.