In vitro lactacystin does not react with the proteasome. It rather undergoes a spontaneous conversion (lactonization) to the active metabolite clasto-lactacystin beta-lactone. Potent and selective irreversible and cell permeable proteasome inhibitor. Inhibits the chymotrypsin-like, trypsin-like and caspase-like peptidase activity of the proteasome. The active metabolite of lactacystin with higher potency since it does not require hydrolysis in order to become cell permeable. Calpain and cathepsin inhibitor. Apoptosis inducer. Anticancer compound. Induces differentiation and inhibits cell cycle progression in several tumor cell lines. Induces neuritogenesis. Autophagy inducer.
Molecular Formula:C10H15NO4
Molecular Weight:213.2
Solubility:Soluble in DMSO or acetonitrile. Undergoes hydrolysis in aqueous buffers.
Storage and Stability:Stable for at least 1 year after receipt when stored at -20°C. Store solutions at -20°C in the dark.
