Adiponectin [ACRP30; AdipoQ] is a promising biomarker of insulin resistance and type 2 diabetes mellitus (T2DM) but also as a potential target for management of the metabolic syndrome. It is a very robust marker that is not prone to degradation or acute inflammatory challenges, is present in relatively high concentrations in the peripheral circulation, and can be collected by a variety of methods. The benefits of using adiponectin assays in clinical settings include, (a) prediction of risk of diabetes and metabolic status and (b) providing a tool to monitor metabolic improvements. Adiponectin exerts anti-atherogenic and anti-inflammatory properties and may be important as a biomarker for obesity-related cardiovascular disease (CVD). New findings showed urinary adiponectin excretion as an independent new biomarker of microvascular and macrovascular damage in T2DM and suggested it as a very promising tool for early cardiovascular disease risk assessment. Adiponectin serum level was also described as a good biomarker of colorectal adenoma, this being related to the positive correlation between obesity and increased risk of cancer at various sites (colorectal, breast, prostate and endometrium).
The Adiponectin BioAssay™ ELISA Kit is to be used for the in vitro quantitative determination of human Adiponectin in serum, plasma, urine and cell culture supernatant. This ELISA Kit is for research use only.
Sensitivity:100pg/ml
Range:0.5-32ng/ml
Specificity:Recognizes human adiponectin. Does not cross-react with mouse adiponectin, rat adiponectin, human resistin, human RELM-beta or human leptin.
Test Principle:A monoclonal antibody specific for adiponectin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, adiponectin is recognized by the addition of a purified polyclonal antibody specific for adiponectin (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of adiponectin in the samples.
Storage and Stability:Store kit at 4°C. After standard reconstitution, prepare aliquots and store at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
