THPO (Thrombopoietin), also known as megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Megakaryocytopoiesis is the cellular development process that leads to platelet production. THPO is a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene.
Source:Recombinant protein corresponding to aa22-353 from human THPO, fused to His-tag at C-terminus, expressed in Insect cells.
Molecular Weight:~36.5kD (340aa), 40-57kD (SDS-PAGE under reducing conditions)
Applications:Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin:<1.0Eu/1 microgram of protein (determined by LAL method)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Insect cell
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
