JMJD6 is also known as bifunctional arginine demethylase and lysyl-hydroxylase JMJD6. JMJD6 acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as u2AF2/u2AF65 and LuC7L2, as a regulator of RNA splicing by mediating 5-hydroxylation of u2AF2/u2AF65, affecting the pre-mRNA splicing activity of u2AF2/u2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code.
Source:Recombinant protein corresponding to aa1-414 from human JMJD6, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight:~50kD (437aa)
Applications:Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 30% glycerol.
