Interleukin 33 (IL-33) is a 32kD proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kD and C-terminal fragment of 18kD by cleavage of caspase-1.
Source:Recombinant protein corresponding to aa109-266 from mouse IL33, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight:~18.1kD (181aa), confirmed by MALDI-TOF
Applications:Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin:<1.0Eu/1 microgram of protein (determined by LAL method)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in PBS, 1mM DTT, 10% glycerol.
