IL2 (Interleukin2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant mouse IL2 was purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Source:Recombinant protein corresponding to aa21-169 from mouse IL2 expressed in E.coli.
Molecular Weight:~17.3kD (150aa), confirmed by MALDI-TOF
Applications:Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin:<1.0Eu/1 microgram of protein (determined by LAL method)
Biological Activity:Measured in a cell proliferation assay using CTLL-2 mouse T lymphocyte. The ED50 for this effect is <0.9ng/ml.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
